What is nanopore sensing technology?
Nanopore sensing is an electrophoretic approach that can identify a single molecule as it transports through a pore of nanometer scale embedded in a lipid or solid-state membrane. The resulting electric signal is exquisitely sensitive to the detected molecule and thus enables access to information about its size, mass, charge, composition, structure and conformation in real-time. This allows the detection of biomolecules rapidly at the single-molecule level in aqueous solution without the need of labels and amplification. Also, nanopores employ an electrical readout that is cheap and can be massively parallelized. To date, it has been applied for sensing various biological and synthetic molecules, including DNA, peptides, proteins, glycans, PEGs, etc.
Nanopores are by far the first class of single-molecule sensors for quantitative analysis. Single molecule sensitivity is achieved by detecting a transient decrease in ionic conductivity through the nanopore caused by the molecule of interest translocates and thus partially blocks the pore.
Fast & Real-time
Different from other sequencing approaches, Nanopores sequencing data are made available during, as well as after, a run which can be analyzed in real-time. Real-time analysis of data provides advantages where time to answer is important.
By analysing the modulation of the ionic current in terms of the blockade amplitude, duration and frequency, nanopores have been applied to the stochastic sensing and characterization of DNA, RNA, peptides, proteins, metabolites, synthetic polymers and protein–DNA complexes at the single-molecule level.
NanoReader is the first in class portable DNA sequencer for on-site, real-time DNA verification.
- Portable (Hand-size)
- Ultra-sensitive (Pico-Femtoampere)
- Stable and robust
NanoCassette is a ready-to-use chip equipped with nanopore sensor and electrode to connect and work with NanoReader.
- Immediate use (No sample preparation required)
On-site, real-time DNA verification for Anti-counterfeiting
Counterfeiting accounted for 2.5% of world trade in which 18% ($500 billion) for the textile industry. The global anti-counterfeiting market is expected to generate revenue of USD 208.71 Billion by the end of 2026. DNA barcoding is considered the most secure approach for tracing and tracking. However, lack of portable device and slow in speed in DNA detection hamper the efficiency and applicability of this method. Check example companies using DNA barcoding on the right.
What is DNA barcoding?
Every DNA strand contains a unique sequence of genetic information. DNA barcoding uses the same underlying idea to combat counterfeiting. It implants a unique DNA tag into a product or package, rendering it traceable, identifiable and verifiable. This technology is compatible with all material types and can therefore be applied to products as diverse as bottles containing alcohol and their labels, perfumes, refined fuels and banknotes.
Textile industry is urgently looking for approaches to trace and track their products, including demin, cashmere, cotton, silk and other materials. DNA tags can serve as invisible and integratable tags to provide product traceability and authentication. NanoDecoder’s devices can provide portable and easy-to-use devices for on-site and real-time verification to improve the supply chain transparency.
Customized DNA tags serve as the identity of each artwork/author to protect the value of their artworks. NanoDecoder devices offer the DNA verification in a fast and easy-to-use way.
“Safe food, Better health”. Food safety, compliance and integrity are of great importance to everyone’s daily life. DNA tags or DNA carried by the product can be traced and tracked to know the food origin. NanoDecoder’s devices provide portable device that can be taken to remote fields, such as farm, to offer immediate verification.
Peptide quality control
Nanopore sensing technology has shown great potential in peptide/protein sequencing. We forsee the first application of nanpore sensing technology is for peptide quality control.
Nanopore technolgy employs the ultra-sensitive ionic current readout. It is a very promising approach for membrane target drug screening.
Our device can detect DNA, RNA, peptide, proteins and their post-translational modification which has a important indication in disease progression.
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